National University Corporation Okayama University
Elucidation of the three-dimensional structure of the PSI-LHCI supercomplex of the red alga Cyanidium caldarium and the molecular evolution of the LHC [Shizuoka University, Okayama University, RIKEN, Tohoku University, Kyoto University, Toyohashi University of Technology]
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This is a press release on joint research between Shizuoka University, Okayama University, RIKEN, Tohoku University, Kyoto University, and Toyohashi University of Technology.
March 11, 2024 (Reiwa 6) National University Corporation Okayama University https://www.okayama-u.ac.jp/
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-Research points-
Single particle structure analysis using cryo-electron microscopy revealed that PSI consists of photosystem I (PSI) and light-harvesting pigment protein (LHC) of the red alga Cyanidium caldarium (hereinafter referred to as C. caldarium), which performs oxygen-evolving photosynthesis. -We have elucidated the three-dimensional structure of the LHCI supercomplex.
We revealed that C. caldarium PSI-LHCI has a molecular structure in which five LHCI subunits are bound to a PSI monomer.
Through molecular phylogenetic analysis of LHCI proteins based on structural analysis of PSI-LHCI, we proposed an evolutionary model for LHCI in the red evolutionary lineage originating from red algae.
◆Research overview
Associate Professor Ryo Nagao of Shizuoka University, Specially Appointed Associate Professor Koji Kato of Okayama University, Professor Kenren Shen, Group Director and Professor Koji Yonekura of RIKEN/Tohoku University, Associate Professor Yu Hamaguchi, PhD student Minoru Kumazawa of Kyoto University, Professor Kentaro Ifuku’s research group, along with unit leader Nao Domae of RIKEN and associate professor Yu Hirose of Toyohashi University of Technology, conducted single particle structure analysis using a cryo-electron microscope (JEOL CRYO ARM 300) to identify red algae. We solved the three-dimensional structure of PSI-LHCI from C. caldarium at a resolution of 1.92 A.
In the obtained PSI-LHCI structure, five LHCIs were bound to the PSI monomer. Furthermore, we focused on LHCI of the purple evolutionary lineage, for which the 3D structure of PSI-LHCI including C. caldarium has been clarified, and proposed an evolutionary model of LHCI based on molecular phylogenetic analysis.
Molecular phylogenetic analysis is the mainstream of research on the evolution of LHCs, but this method alone cannot identify which LHCs bind to PSI. In this study, we identified the LHCI that binds to PSI through 3D structural analysis, and performed molecular phylogenetic analysis focusing only on the identified LHCI. This approach has made it possible to understand the molecular evolution of LHCI in the purple lineage from an unprecedented angle. In this way, it is expected that the combination of 3D structure analysis and molecular phylogenetic analysis will create a new trend in research on the evolution of LHCI.
The results of this research were published in the Proceedings of the National Academy of Sciences of the United States of America on March 5, 2024.
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[Image 3: https://prtimes.jp/i/72793/2048/resize/d72793-2048-5e5ab8f4ee498b3c16fe-2.jpg&s3=72793-2048-44b88798c1cc08c41ee57efe18d3e238-573×520.jpg] ◆Researcher Comment: Ryo Nagao, Associate Professor, Faculty of Agriculture, Shizuoka University This research aims to explore new research directions that approach the evolution of the LHC by combining the specialized knowledge and techniques of each group. I was able to show it. I would like to continue to explore the evolution of the LHC by using this research infrastructure.
◆Paper information Journal name: Proceedings of the National Academy of Sciences of the United States of America Paper title: The structure of PSI-LHCI from Cyanidium caldarium provides evolutionary insights into conservation and diversity of red-lineage LHCs Author: Koji Kato, Tasuku Hamaguchi, Minoru Kumazawa, Yoshiki Nakajima, Kentaro Ifuku, Shunsuke Hirooka, Yuu Hirose, Shin-ya Miyagishima, Takehiro Suzuki, Keisuke Kawakami, Naoshi Dohmae, Koji Yonekura, Jian-Ren Shen, Ryo Nagao DOI: https://www.pnas .org/doi/10.1073/pnas.2319658121 ◆About detailed research content
Elucidation of the three-dimensional structure of the PSI-LHCI supercomplex of the red alga Cyanidium caldarium and the molecular evolution of LHC
https://www.okayama-u.ac.jp/up_load_files/press_r5/press20240308-1.pdf
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◆Contact information for this matter
-Research-related Ryo Nagao, Associate Professor, Faculty of Agriculture, Shizuoka University TEL: 054-238-4251 Summary of websites and SNS (QR code): https://linktr.ee/ryonag RIKEN Synchrotron Radiation Scientific Research Center Group Director Koji Yonekura (Professor, Institute of Multidisciplinary Materials Science, Tohoku University) Kenjin Shin, Professor, Institute for Interdisciplinary Basic Science, Okayama University – Matters related to the press – Shizuoka University Public Relations and Funds Division TEL: 054-238-5179 Okayama University General Affairs and Planning Department Public Relations Division TEL: 086-251-7292 RIKEN Public Relations Office Press Office TEL: 050-3495-0247 Tohoku University Institute of Multidisciplinary Materials Science Public Relations Information Office TEL: 022- 217-5198 Kyoto University Public Relations Division, International Public Relations Office TEL: 075-753-5729 Toyohashi University of Technology General Affairs Division Public Relations Section TEL: 0532-44-6506
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