MTG Co., Ltd.
Elucidating the effective frequency band for myokine expression. Research results published in “Journal of Comparative Physiology B” ……
MTG Co., Ltd. (Headquarters: Nagoya City, Aichi Prefecture, President and CEO: Tsuyoshi Matsushita) and a group led by Yuichi Nishikawa of Kanazawa University have identified a frequency band that is effective for the expression of myokine.
Myokine is a general term for substances expressed in skeletal muscle, and more than 600 types of myokine candidates have been identified to date. Among these, brain-derived neurotrophic factor (BDNF) and cathepsin B are known as substances that contribute to neuroprotection and improvement of cognitive function. Animal studies have shown that these substances are expressed in large quantities through
high-intensity exercise, and that they also contribute to improving cognitive function, demonstrating the importance of exercise in preventing dementia and improving symptoms. has been attracting attention in recent years.
A research group with Assistant Professor Nishikawa and colleagues has previously reported that BDNF in the blood can also be increased by using a method called neuromuscular electrical stimulation (EMS), which applies electrical stimulation to muscles to cause them to contract. However, EMS has various variable parameters such as frequency and pulse width, and it was not clear whether such stimulation is effective for myokine expression.
In this study, we stimulated muscles at different frequencies and examined which frequency band was most effective for myokine expression.
As a result, it was revealed that stimulation at 20Hz had a higher expression level than other frequency bands (4Hz and 80Hz).
It is expected that this knowledge will be applied to intervention methods aimed at improving cognitive function in the future. The results of this research were published in “Journal of Comparative Physiology B” at 0:00 on May 31, 2024 (London time).
*Electrical Muscle Stimulation=Skeletal muscle electrical stimulation ■Purpose
Quantifying the optimal EMS frequency to stimulate myokine production ■Method
The subjects were 16 young adults, and the intervention was conducted for 20 minutes each on separate days under four conditions: 4Hz, 20Hz, 80Hz, and control. Blood was collected before and after EMS
intervention, and changes in cathepsin B and BDNF were confirmed. In the control condition, blood was collected before and after 20 minutes of resting sitting.
■Results
Stimulation at 20Hz significantly increased cathepsin B and BDNF concentrations after intervention compared to before intervention. Furthermore, stimulation at 20 Hz significantly increased cathepsin B and BDNF concentrations compared to stimulation at 4 Hz, 80 Hz, and control conditions.
[Example of results]
[Image 1: https://prtimes.jp/i/29736/599/resize/d29736-599-ff956896c6e4f2b2dab0-0.jpg&s3=29736-599-fc35332ace35de19ffcf7738f5507906-768×291.jpg] *p<0.05
[Image 2: https://prtimes.jp/i/29736/599/resize/d29736-599-48aa00cad92848e3586b-1.jpg&s3=29736-599-a8f67bc72457fc793c27e697e1ce65b4-775×293.jpg] *p < 0.05, , **p < 0.01, ***p < 0.001
■Conclusion
It was suggested that stimulation at 20Hz effectively evoked cathepsin B and BDNF responses.
Click here for the content of the paper
https://link.springer.com/article/10.1007/s00360-024-01566-0?utm_source=rct_congratemailt&utm_medium=email&utm_campaign=oa_20240531&utm_content=10.1007/s00360-024-01566-0 ■Title
Influence of stimulation frequency on brain-derived neurotrophic factor and cathepsin-B production in healthy young adults
■Author
Yuichi Nishikawa 1, Hiroyuki Sakaguchi 2, Tatsuya Takada 3, Noriaki Maeda 4, Allison Hyngstrom 5
1 Faculty of Frontier Engineering, Institute of Science & Engineering, Kanazawa University, Kakuma-machi, Kanazawa, Ishikawa 920-1192, Japan 2 Graduate School of Frontier Engineering, Kanazawa University, Kanazawa, Japan 3 MTG Co., Ltd., Nagoya, Japan
4 Department of Sport Rehabilitation, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan
5Department of Physical Therapy, Marquette University, Milwaukee, WI, USA MTG not only develops our own products, but also engages in
industry-government-academia joint research with universities, as well as joint development with other companies, to create innovative brands by fusing cutting-edge technology and the highest level of wisdom. I have come. We will continue to provide products and services that help people around the world live vibrant, positive lives, and support the creation of healthy bodies for many people.