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Home » U-Factor Co., Ltd. Discovery of a component derived from immortalized stem cells that has the same effect as stem cell transplantation

U-Factor Co., Ltd. Discovery of a component derived from immortalized stem cells that has the same effect as stem cell transplantation

[U-Factor Co., Ltd.] Discovery of a component derived from
immortalized stem cells that has the same effect as stem cell transplantation
*U-Factor Co., Ltd.*
Press release: July 11, 2024
**
Discovery of ingredients derived from immortalized stem cells that have the same effect as stem cell transplants
*High expectations as a new therapeutic drug to support
next-generation medical care*
*Points*
– The culture supernatant of stem cells derived from deciduous dental pulp contains components that improve cell activity.
– Cytokines in the culture supernatant of stem cells enhance migration ability and possess wound healing activity.
– Stem cell culture supernatant components have the effect of increasing antioxidant activity.
– It is attracting attention as a pharmaceutical composition that has the potential to bring innovation to future medical care. Diagram of the purification process from deciduous teeth to extraction of culture supernatant components
* overview *
U-Factor Co., Ltd. (hereinafter referred to as “our company”) is a member of the Cellular and Molecular Engineering Research Division of the National Institute of Advanced Industrial Science and Technology (hereinafter referred to as “AIST”).
In collaboration with Senior Principal Researcher Yuji Teramura, we discovered that purified components of culture supernatant produced from immortalized human deciduous tooth-derived stem cells (SHED) improve cell activity and migration ability, and retain antioxidant activity. I discovered that. Since this result retains the effects of stem cell transplantation, it is expected that this technology can be used as an alternative to stem cell transplantation, providing a safer and more efficient treatment method.

The details of this technology were published in “Scientific Reports” on July 3, 2024.
* Social background of development *
In recent years, treatments using stem cells have been attracting attention in the field of regenerative medicine, but stem cell transplants come with the risks of immune rejection and tumor formation, making balancing safety and efficacy an issue. Against this background, there is a need for new treatment methods that use culture supernatants produced from stem cells. Stem cell-derived culture supernatant contains multiple cytokines and is expected to promote cell regeneration and repair. However, there are many unknowns regarding its safety, quality, and effectiveness, and especially when using primary cultured cells, there are problems such as limits on cell proliferation, the amount of culture supernatant produced, and contamination with impurities. Exists. There is an urgent need to overcome these challenges and develop a safe and effective culture supernatant.

* Research history *
Minoru Ueda, director of our company and professor emeritus at Nagoya University Graduate School of Medicine, has been conducting research on culture supernatants containing cytokines produced by stem cells for many years (K
Matsubara, M Ueda, et al. * J. Neurosci*
2015). This research has confirmed that culture supernatant liquid is effective against spinal cord injury and acute organ injury. However, when using primary cultured cells, there are limits to cell
proliferation and the amount of culture supernatant produced. To solve this problem, we aimed to produce a more stable supply and higher quality culture supernatant using immortalized deciduous tooth-derived dental pulp stem cells.

*Research content*
In this study, we conducted an evaluation test using various cells to evaluate the effects of the culture supernatant solution of
immortalized deciduous tooth-derived dental pulp stem cells
(hereinafter referred to as supernatant stock solution) and its extracted components (hereinafter referred to as extracted
components). I did it. Compare the supernatant stock solution and its extracted components from the viewpoints of intracellular
dehydrogenase activity, migration activity, and antioxidant stress activity, and obtain results that the extracted components have the same or higher activity as the culture supernatant of primary cultured cells. I was able to do it.

*1. Dehydratase activity experiment *
In this experiment, we added the culture supernatant to mouse-derived fibroblasts (NIH3T3 cells) and evaluated the effect of the culture supernatant. The supernatant stock solution and extracted components were added at various concentrations and the degree of cell activity was measured. The graph on the left shows the results for primary cultured cells, and the graph for extracted components (red line) is increasing, indicating that the cell activity is more active than the supernatant solution (black line) and other extracted components (blue and yellow lines). I understand that it is high. The graph on the right shows the results for the supernatant stock solution, and it was found that the results were similar to the graph for primary cultured cells.
Results of dehydratase activity experiment
*2. Scratch assay experiment *
This is an experiment to evaluate the migration ability (migration ability) of cells. A scratch is made on the cultured cell layer, the supernatant stock solution or extracted components are added, and the extent to which the cells fill the scratch is observed. “Cell” measures the speed and area at which cells fill wounds
recovery
area (%). This experiment measures how well different supernatants promote cell migration and repair. The graph on the right shows that the cell migration ability of extract component 1 is equivalent to that of supernatant solution (CM).
Scratch assay experiment results
*3. Oxidative stress experiment *
This experiment evaluates cell damage caused by hydrogen peroxide treatment to confirm the resistance of cells to oxidative stress. From the graph on the right, it was found that the supernatant solution and extract component 1 were able to suppress cell damage caused by hydrogen peroxide treatment. No inhibitory effect on cell damage was confirmed with other extract components. These results confirmed that the supernatant solution and extracted components suppressed cell damage caused by oxidative stress.
Oxidative stress experiment results

Furthermore, compared to the supernatant liquid, impurities such as ammonia and lactic acid were removed from the extracted components. At the same time, since the extracted components are rich in cytokines (VEGF, HGF, bFGF, etc.) that are considered to be active ingredients, a high-quality supernatant liquid is created in which the active ingredients function fully without impurities. was achieved. This is expected to significantly improve the safety and effectiveness of treatment.

* Future plans *
In the future, we will further advance the development of specific treatment methods using extracted ingredients. We aim to pursue further innovation in the field of regenerative medicine and provide new treatment options that improve the quality of life of patients. Through future research and clinical trials, we will confirm safety and effectiveness and take steps toward practical application. Ultimately, we aim to bring benefits to many patients through our technology, and we aim to introduce it to medical settings as soon as possible.
* Paper information *
Magazine name: Scientific Reports
Paper publication date: July 3, 2024
Paper title: Activation of cellular antioxidant stress and migration activities by purified components from immortalized stem cells from human exfoliated deciduous teeth
Author: Yujing Shu, Masato Otake, Yasuhiro Seta, Keigo Hori, Akiko Kuramochi, Yoshio Ohba, Yuji Teramura
DOI: 10.1038/s41598-024-66213-8

– Glossary
*Human deciduous tooth-derived stem cells (SHED)*
Stem cells extracted from baby teeth. These cells have high
proliferative and differentiation abilities and are attracting attention in regenerative medicine and tissue repair research. SHED can be differentiated into a variety of cells such as nerves, bones, and muscles, so it has the potential to be applied to a variety of treatments in the future.

* Immortalization *
It refers to a technology that allows cells to multiply indefinitely without aging or dying naturally. After a normal cell divides a certain number of times, it ages, loses its function, and eventually dies. However, by using immortalization technology, cells can avoid this aging process and continue to divide and proliferate. This technology is extremely useful in research and medical fields, and is particularly widely used in regenerative medicine and cancer research. *About details about this release*
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