[National University Corporation Kumamoto University] New testing method for the new coronavirus mutant virus using a simultaneous multi-item measuring device – A new molecular diagnostic method that can respond to the evolution of the virus –
*National University Corporation Kumamoto University*
Press release: August 19, 2024
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A new testing method for the new coronavirus mutant virus using a simultaneous multi-item measuring device – A new molecular diagnostic method that can respond to the evolution of the virus –
[Point]
Next-generation simultaneous multi-item detection system IntelliPlex*1 and calculation program OVI (Objective Variant
We have developed a novel mutant virus identification system “Intelli-OVI” that combines the following:
Testing using Intelli-OVI enables rapid and highly accurate
classification of over 20 different SARS-CoV-2 mutant viruses. This new generation molecular diagnostic tool is expected to help prepare for future pandemics and contribute to the realization of a safe and secure society.
[Summary explanation]
Professor Kenfumi Sato of the Joint Research Center for Human Retrovirology, Kumamoto University Campus, a joint research group of the Tokyo Metropolitan Health Safety Research Center and Kumamoto University of Health Sciences, has developed a new coronavirus mutated virus diagnostic tool, Intelli-OVI.
[Future development]
Using the example of the new coronavirus, the results of this study demonstrate that Intelli-OVI can respond to rapidly evolving viruses, and are expected to be applied to diagnostic tools for various viral infections, including future pandemic viruses. will be done. We will continue to conduct further research and development using this new-generation molecular diagnostic tool to prepare for the emergence of new emerging and re-emerging viruses and to realize a safe and secure society.
The results of this research were published in the British scientific journal “Communications” on August 9, 2020.
Published in “Medicine.” This research was carried out by the Japan Agency for Medical Research and Development (AMED), an international science and technology joint research and development promotion project in the medical field.
Strategic International Collaborative Research Program (SICORP) Japan-UK joint research “Human T-cell leukemia virus type 1: Analysis of diversity of viral gene expression and elucidation of its control mechanism by single cell analysis” (JP22jm0210074), Denka Co., Ltd. It was done with support.
[Term explanation]
*1: IntelliPlex
A highly sensitive multi-item simultaneous measurement system developed by PlexBio.
By combining the πCode method (described later) and fluorescence measurement technology, we have achieved both high sensitivity and simultaneous multi-item measurement.
The πCode method is a technology that uses a new labeling method to immobilize antibodies and DNA probes on magnetic microdisks to identify the type of test target and enable simultaneous measurement of multiple items.
In June 2021, Denka Corporation began selling a detection research reagent that uses IntelliPlex to detect mutation sites in the then-popular virus.
In addition, IntelliPlex and π code are trademarks of PlexBio in Japan. *2: OVI (Objective Variant Identification)
A computational algorithm for analyzing data obtained with IntelliPlex and identifying multiple mutant strains simultaneously.
[Paper information]
Paper title: A micro-disc-based multiplex method for monitoring emerging SARS-CoV-2
variants using the molecular diagnostic tool Intelli-OVI
Author: Md Belal Hossain, Yoshikazu Uchiyama, Samiul Alam Rajib, Akhinur Rahman, Mitsuyoshi Takatori, Benjy Jek Yang Tan, Kenji Sugata, Mami Nagashima, Mamiyo Kawakami, Hitoshi Ito, Ryota Kumagai, Kenji Sadamasu, Yasuhiro Ogi, Tatsuya Kawaguchi, Tomokazu Tamura, Takasuke Fukuhara, Masahiro Ono, Kazuhisa Yoshimura & Yorifumi Satou.
Journal: Communications Medicine
DOI: 10.1038/s43856-024-00582-z
URL: https://pubmed.ncbi.nlm.nih.gov/39122992/
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https://www.kumamoto-u.ac.jp/whatsnew/seimei-sentankenkyu/20240819